Test: Vitamin D,25-OH

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Background/Purpose

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia

Procedure

Patient Preparation
Fasting preferred, but not required.

Preferred Specimen(s)
0.8 mL serum

Minimum Volume
0.5 mL

Specimen Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 60 days

Reject Criteria
Gross hemolysis • Gross lipemia • Plasma

Methodology
Immunoassay (IA)
Performed: Bilaterally

Dialog

Blood level testing for Vit D levels

Criteria

• 30-100 ng/mL

Measurement

30 ng/mL

LOINC Code

0

Scale/Grading

Pass/Fail